erk1 2 mapk pathway targeting mek1 2  (MedChemExpress)

Journal: Life (Basel, Switzerland)

Article Title: Targeting MAPK Signaling: Loureirins A and B from Dracaena Loureiri Inhibit Epithelial-Mesenchymal Transition and Invasion in Non-Small Cell Lung Cancer Cell Lines.

doi: 10.3390/life15030396

Figure Lengend Snippet: Figure 7. Loureirin A and Loureirin B suppressed the activation of the MAPK signaling pathway in A549 and H1299 cells. A549 (A,C) and H1299 (B,D) cells were treated with 0–140 µM Loureirin A or B for 48 h. Phosphorylation of ERK, JNK, and p38 was assessed by Western blotting. The fold change of phosphorylated/total proteins in the control group (0 µM) was set to 1 to determine the relative fold change in the treatment groups. Results are expressed as mean ± S.D. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with a control group (0 µM).

Article Snippet: The primary antibodies for Western blot analysis, used to detect invasive proteins (u-PA, u-PAR, and MT1-MMP) and EMT markers (fibronectin, N-cadherin, and vimentin) and MAPK signaling pathway (p-ERK1/2 (cat. no. 4377S), ERK1/2 (cat. no. 4696S), p-JNK (cat. no. 9255S), JNK (cat. no. 9252S), p-p38 (cat. no. 4631S), and p38 (cat. no. 9212S)) protein expression, and the secondary antibodies, namely goat anti-mouse (cat. no. 7076S) or rabbit (cat. no. 7074S) IgG conjugated with horseradish peroxidase, were obtained from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Activation Assay, Phospho-proteomics, Western Blot, Control

Journal: PLOS Neglected Tropical Diseases

Article Title: A novel Trichinella spiralis serine proteinase disrupted gut epithelial barrier and mediated larval invasion through binding to RACK1 and activating MAPK/ERK1/2 pathway

doi: 10.1371/journal.pntd.0011872

Figure Lengend Snippet: A : rTsSPc up-regulated expression of RACK1. After Caco-2 cells were treated with rTsSPc, trypsin or IIL ES antigens, the RACK1 expression was obviously up-regulated compared to the PBS group. B : rTsSPc up-regulated expression of p-ERK1/2. ERK1/2 phosphorylation level in Caco-2 cells treated with rTsSPc was detected by Western blot. Compared to the PBS group, the ERK1/2 MAPK signaling pathway was activated and ERK1/2 phosphorylation level was evidently increased in Caco-2 cells treated with rTsSPc, trypsin and IIL ES antigens. ΔrTsSPc represents the heating inactivated rTsSPc at 100 °C for 5 min, and * in the figure represents P < 0.0001 compared to the PBS group. Each experiment was carried out in triplicate.

Article Snippet: To confirm that rTsSPc binding to RACK1 damaged the TJs integrity through activating ERK1/2 MAPK pathway and down-regulating expression of TJs proteins, RACK1 inhibitor harringtonolide (HO; 40 μM; MedChemExpress, USA) and ERK1/2 pathway inhibitor PD98059 (20 μM; MCE, USA) were used in this study [ , ].

Techniques: Expressing, Phospho-proteomics, Western Blot

Journal: PLOS Neglected Tropical Diseases

Article Title: A novel Trichinella spiralis serine proteinase disrupted gut epithelial barrier and mediated larval invasion through binding to RACK1 and activating MAPK/ERK1/2 pathway

doi: 10.1371/journal.pntd.0011872

Figure Lengend Snippet: Caco-2 monolayer was pretreated with two inhibitors HO and PD98059, and then incubated with rTsSPc, the ERK1/2 pathway was not activated in inhibitors-pretreated Caco-2 cells. A : Western blotting analysis of RACK1 expression level in Caco-2 cells. B : Western blotting analysis of ERK1/2 expression level in Caco-2 cells. Quantitative analysis of protein bands in Western blot was performed by using ImageJ software. ΔrTsSPc represents the heating inactivated rTsSPc. Each experiment was performed in triplicate. * in the figure represents P < 0.05 compared to the PBS group. # P < 0.05 compared to the rTsSPc group; ♥ P < 0.05 relative to the IIL ES antigen group.

Article Snippet: To confirm that rTsSPc binding to RACK1 damaged the TJs integrity through activating ERK1/2 MAPK pathway and down-regulating expression of TJs proteins, RACK1 inhibitor harringtonolide (HO; 40 μM; MedChemExpress, USA) and ERK1/2 pathway inhibitor PD98059 (20 μM; MCE, USA) were used in this study [ , ].

Techniques: Incubation, Western Blot, Expressing, Software

Journal: PLOS Neglected Tropical Diseases

Article Title: A novel Trichinella spiralis serine proteinase disrupted gut epithelial barrier and mediated larval invasion through binding to RACK1 and activating MAPK/ERK1/2 pathway

doi: 10.1371/journal.pntd.0011872

Figure Lengend Snippet: A : Western blotting of expression of RACK1 and p-ERK in infected mouse intestine. B : HO and PD98059 inhibited the RACK1 expression in infected mice. C : HO and PD98059 decreased up-regulation of p-ERK1/2 in infected mice. Each test had three replicates. * P < 0.0001 compared to the uninfected PBS group; # P < 0.001 relative to the infected PBS group; ♥ P < 0.05 compared to the only HO or PD98059 alone group.

Article Snippet: To confirm that rTsSPc binding to RACK1 damaged the TJs integrity through activating ERK1/2 MAPK pathway and down-regulating expression of TJs proteins, RACK1 inhibitor harringtonolide (HO; 40 μM; MedChemExpress, USA) and ERK1/2 pathway inhibitor PD98059 (20 μM; MCE, USA) were used in this study [ , ].

Techniques: Western Blot, Expressing, Infection